The Genomics Core is home to state-of-the-art equipment and expert staff who provide a range of genomics services to internal and external users. Please email genomics@vai.org for more information.

Illumina NextSeq 500
A flexible sequencing system appropriate for small/mid-range RNAseq, ChIPseq and other next-generation sequencing applications

Illumina NovaSeq 6000
A high-throughput system for large-scale genomic, epigenomic and transcriptomic studies.

Illumina iScan with Tecan Freedom EVO
A high-throughput genotyping system that can assay 288 individuals in a single run. Assays include:

  • Infinium EPIC methylation array
  • QC array
  • MultiEthnicGlobal array
  • Omni SNP genotyping arrays

10X Genomics Chromium
A nano droplet-based library preparation system that is useful for high-throughput single cell 3′ RNA expression analysis of single cell ATAC-seq and single cell immune¬†profiling. Linked-read DNA analysis can provide structural and CNV information from genomics samples.

Shared equipment for walk-up use
The Genomics Core also provides a suite of shared equipment for walk-up use for well-trained, internal users. For questions or to set up training, please email genomics@vai.org.

Covaris LE220 Sonicator
Optimized for high-throughput, pre-analytical sample processing, including:

  • DNA shearing for next-gen sequencing
  • Cell lysis
  • Tissue disruption
  • Compound dissolution

BioRad CFX384 Well qPCR System
Offers a 384-well format with four-color detection optics for singleplex or multiplex reactions.

Qubit 2.0 Fluorometer
Provides accurate and sensitive DNA, RNA, and protein quantitation that is ideal for low concentration samples and samples entering next-gen sequencing library preparation.

Advanced Analytical Fragment Analyzer
Provides DNA and RNA quality and integrity analysis in a high throughput manner. Up to 288 samples can be analyzed at once.

BioRad Droplet Digital PCR
Absolute quantification of DNA or RNA PCR targets at single-copy resolution. Useful for:

  • Rare target enrichment
  • Copy number variation
  • Mutation detection
  • Gene expression analysis
  • DNA methylation detection