Resources

Please see the below PDFs for guidelines on genomics best practices and core policies.

ChIP-Seq submission recommendations

Customer-prepared library submission guidelines

Genomic DNA, targeted sequencing and bisulfate sequencing submission guidelines

iScan policies

NextGen sequencing policies

RNA submission guidelines

Illumina Sequencing (KAPA QPCR, hyperprep, totalRNA, mRNA protocols)

• Sequencing workflow (video)
• Updated 3D SBS video (video)

Illumina Library Preparation

• KAPA Hyper Prep Kits (product information)
  • KAPA Hyper Prep Kit technical data sheet
• KAPA ribosomally reduced RNA (product information)
  • KAPA Hyper Prep Kit technical data sheet
• KAPA stranded mRNA (product information)
  • KAPA Stranded mRNA-seq Kit technical data sheet
• KAPA Library qPCR Quantification (product information)
  • KAPA Library Quantification Kit technical data sheet

Illumina iScan

• List of assay types
• iScan Infinium Technology (video)

10x Chromium Single Cell

• Cell Prep Guide
• Sample and Library Preparation

10x Chromium Long Read

• High-molecular weight DNA extraction from fresh frozen tissue
• Genome and exome library preparation guides 

Quantification/Qualification

• Nanodrop Assessment of DNA and RNA purity
• PicoGreen Fluorometric Quantitation
• PicoGreen Fluorometric Quantification with Biotek Plate Reader
• Qubit Fluorometric Quantification
• Quantifluor (Promega) Fluorometric Quantification
• RNA yield from standard tissue types
• SPRI bead cleanups (video)

Covaris LE220 Sonicator

• Consumables ordering page
• Quick guide

PacBio

• SMRT sequencing overview (video)
• SMRT sequencing overview 2 (video)
• SMRTbell Libraries

BioRad QX200 and Autoloader (product information)

• Droplet Digital PCR applications guide