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Services

Genomics

Services

The Genomics Core prides itself on conducting high-caliber science and providing exceptional customer service. For questions and pricing information, or to submit a service request, please email genomics@vai.org. For more information on requesting services, please scroll down.

SEQUENCING

10X Chromium single cell RNA sequencing
Ideal for measuring expression levels and dissecting cell types in thousands of single cells in a cost-effective manner.

10X Chromium linked read sequencing
Provides pseudo-long read whole genome and targeted sequencing to help resolve large scale genomic architecture and scaffolding, long-range haplotyping and structural variant detection.

Whole-genome sequencing
Whole-genome sequencing gives scientists the ability to comprehensively study the genome by providing a complete look at an organism’s genetic information.

Custom targeted sequencing
Targeted sequencing allows scientists to isolate and sequence a specific set of genes or areas of the genome, reducing cost and time to results as well as providing a more precise data set.

Exome sequencing
The majority of disease-associated variants are found in the exome, the region of the genome responsible for protein-coding. A type of targeted sequencing, exome sequencing gives scientists the ability to focus on genes that are more likely to be associated with a phenotype of interest while providing a faster and more cost effective alternative when whole-genome sequencing is not required.

Methylation sequencing
Focusing on CpG and enhancer-rich regions of the genome, methylation sequencing is aimed at determining epigenetic information in a more targeted, cost effective manner, when whole genome information is not necessary

Bisulfite sequencing (RRBS/WGBS)
Bisulfite sequencing interrogates the epigenetic information contained and mediated by cytosine modifications such as methylation (5mC) and hydroxymethylation (5hmC). Both reduced-representation and whole genome bisulfite sequencing options are available.

RNA-seq
RNA-seq is a precise method for analyzing the transcriptome, and provides quantitative as well as qualitative data.

ChIP-Seq
IP sequencing allows researchers to look at the specific extrachromosomal modifications (transcription factors, histone modifications) that affect gene accessibility, transcription and translation.

RIP-seq
Sequencing of actively transcribed regions of the RNA polymerase complex allows researchers to assess actively transcribed regions under specific growth conditions.

GENOTYPING

Illumina Bead Arrays
Using the iScan platform, the Illumina bead arrays allow for cost-effective interrogation of millions of unique genomic sites. Commonly run array types include: QC array, EPIC methylation array. Projects are expedited by automation on the Tecan Freedom EVO liquid handler

Real-time PCR
A staple of biomedical research, real-time PCR amplifies and analyzes specific RNAs to aid in the study of gene expression.

Digital droplet PCR
Much like real-time PCR, digital droplet is used to study gene expression but has the added ability to partition samples on a large scale while also requiring less sample volume.

HOW TO REQUEST SERVICES

Prior to requesting genomics services, please review the appropriate submission policies found here. All requests should be submitted via email to genomics@vai.org along with the completed, requisite form, which may be found below. Core staff will add the service request to CrossLab.

VARI staff that need a login (or further CrossLab support) should contact Lori Moon and MSU staff that need a login should contact Erica Lange. External users that need an CrossLab account/login can go here for account creation. Current CrossLab users can login here. After logging in, external users should click “list all cores” under the Core Facilities section in the left menu and scroll down to “Cores at Van Andel Institute”.

For more information, please email genomics@vai.org.

Submission forms

Please ensure the appropriate form is completely filled out and included with your service request. Completed forms should be sent to genomics@vai.org.

iScan Array Submission Form
Sequencing Submission Form