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Fluorescence In Situ Hybridization (FISH)

Genomics

FISH (Fluorescence In Situ Hybridization) is a molecular cytogenetic technique that uses fluorescently labeled DNA probes to target genes or chromosomal regions of interest in order to detect copy numbers (gain, loss, amplification) and/or the location of the specific DNA sequence within the interphase nucleus or metaphase chromosomes. FISH can be used to detect or confirm aneuploidy, microdeletions, inversions, duplications, amplification, chromosomal rearrangements and to refine chromosomal breakpoints. One big advantage of FISH is that non-dividing cells can be used for determining copy numbers and the presence of gene amplification. This is a highly informative technique since many genetic disorders and cancer types have known chromosomal abnormalities that can be easily identified by this method.

What We Offer
  • Custom FISH probe production
  • Dual or Tri-color FISH – Fluorophores: Aqua, green and orange with DAPI (blue) counterstain
  • Centromere, whole chromosome or arm painting, telomere and locus specific FISH
  • Determination of amplification type (intrachromosomal or extrachromosomal double minutes)
  • Species identification to rule out cross-species contamination during cell culture or xenograft experiments
  • Analysis of human, mouse, rat and dog samples
  • Metaphase and/or interphase analysis of cells that grow in culture
  • Interphase analysis on properly fixed samples such as frozen tumors and tissue section slides
Service Includes
  • Cell culture, harvest and slide preparation
  • Analysis of 200 nuclei for interphase FISH and 20-50 cells for metaphase FISH
  • A detailed report and representative images