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G-banding

Genomics

G-banding is a traditional karyotyping method in which trypsin digestion and Leishman stain are used to produce alternating light and dark bands on metaphase chromosomes, with each chromosome displaying a unique banding pattern. The chromosomes can then be counted and characterized to determine the karyotype of a cell. Some limitations of this technique are the inability to detect submicroscopic abnormalities less than 5 Mb in size and characterizing chromosomes of cell lines which are complex. However, it is a fast and low-cost technique to determine chromosome number, aneuploidy, structural rearrangements and receive an overview of the sample’s karyotype.

What We Offer
  • Analysis of human, mouse and rat samples
  • Validation of normal cells or cell lines
  • Analysis of cells from low to moderate complexity
Service Includes
  • Cell culture, harvest and slide preparation
  • Analysis of 20-30 metaphases